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HEK293T cells werefefab cotransfected with pCF-BBtr, the empty vector pcDNA, or pcfeA3Z2b-HA, and increasing amounts of plasmids encoding either wt or mutant Bet (Figure。

Briefly, FeFAB cells are the modified CRFK cells that express β-galactosidase under the control of the FFV LTR, which is trans-activated by the FFV Tas protein in relation to。

FeFAB assay indicated that the culture supernatantfefabs collected from the infected and drug-pretreated cells contained infectious FFV virions, which induce expression of the β-galactosidase in the ...

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